Coding

Part:BBa_K1809025:Design

Designed by: David Morgan   Group: iGEM15_WPI-Worcester   (2015-08-26)


TmAFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 91
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized the sequence for E. coli and to minimize repetitive sequences; removed illegal restriction sites.


Source

Taken from the mealworm beetle Tenebrio molitor. Pubmed ID: 17443386

References